goat α transferrin receptor  (R&D Systems)

Journal: eLife

Article Title: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer

doi: 10.7554/eLife.44594

Figure Lengend Snippet:

Article Snippet: Using rat anti-strain Lai-IgG, rabbit anti-Rab5 or Rab11-IgG (Cell Signaling) or goat anti-transferrin receptor (TfR)-IgG (Santa Crus) as the primary antibody, AlexaFluor594-conjugated donkey anti-rat-IgG, AlexaFluor488-conjugated donkey anti-rabbit or goat-IgG or AlexaFluor405-conjugated donkey anti-rabbit-IgG (Abcam) as the second antibody, Lep-vesicle-Rab5 or Lep-vesicle-Rab11/TfR co-localization were detected by confocal microscopy (402/421 nm excitation/emission wavelengths for AlexaFluor405 detection) and the yellow or white FI reflecting the co-localization was measured as above.

Techniques: Control, Fluorescence, Western Blot, Bicinchoninic Acid Protein Assay, Negative Control

Journal: eLife

Article Title: Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer

doi: 10.7554/eLife.44594

Figure Lengend Snippet:

Article Snippet: Antibody , Goat anti-transferrin receptor (TfR)-IgG , Santa Cruz Biotechnology , Cat#sc-7087 , IF(1:100); WB(1:500).

Techniques: Control, Fluorescence, Western Blot, Bicinchoninic Acid Protein Assay, Negative Control

Journal: PLoS ONE

Article Title: pTyr 421 Cortactin Is Overexpressed in Colon Cancer and Is Dephosphorylated by Curcumin: Involvement of Non-Receptor Type 1 Protein Tyrosine Phosphatase (PTPN1)

doi: 10.1371/journal.pone.0085796

Figure Lengend Snippet: ( A ) Table with 13 plasma membrane-associated proteins identified by QTOF-MS/MS as significantly decreased in T84 cell monolayers treated with curcumin. Data shows average number unique peptides identified from three different experiments ± SD. ( B ) Quantitative analysis of cortactin expression in the T84 cells by M/S. The spectrum values were obtained from three different experiments. The quantification data for cortactin protein was derived from M/S using Scaffold proteome software (version Scaffold_3.1.2). Data are means ± SE, * p <0.05 compared with untreated cells, Student's t test. ( C ) Confirmation of CTTN protein expression by western blotting with biotinylated cell surface protein fraction prepared from T84 cells treated with DMSO (CTRL) or 50 µM curcumin for 1–4 hours. CD71 (transferrin receptor) was used as a loading control. CTRL represents cells treated with DMSO for 4 hours.

Article Snippet: Even loading of biotinylated surface proteins was confirmed with goat polyclonal anti-transferrin receptor antibody (Santa Cruz).

Techniques: Clinical Proteomics, Membrane, Tandem Mass Spectroscopy, Expressing, Derivative Assay, Software, Western Blot, Control

Journal: PLoS ONE

Article Title: pTyr 421 Cortactin Is Overexpressed in Colon Cancer and Is Dephosphorylated by Curcumin: Involvement of Non-Receptor Type 1 Protein Tyrosine Phosphatase (PTPN1)

doi: 10.1371/journal.pone.0085796

Figure Lengend Snippet: HCT116 cells were treated with 50 µM curcumin for 15 min and pre-cleared lysates were immunoprecipitated using anti- pTyr 421 -CTTN or anti-CTTN (total) rabbit polyclonal antibodies. Immunoprecipitated complexes were analyzed by Western blotting for the presence of CTNND1. Lower three panels demonstrate even input of CTTN, CTNND1 and GAPDH in the cell lysates used for co-immunoprecipitation. All images representative of three independent experiments.

Article Snippet: Even loading of biotinylated surface proteins was confirmed with goat polyclonal anti-transferrin receptor antibody (Santa Cruz).

Techniques: Immunoprecipitation, Western Blot